产品简称

serum, from rabbit

NACRES

NA.41

抗体产品类型

primary antibodies

物种反应性

human

生物来源

rabbit

单抗克隆号

polyclonal

抗体应用

western blot: suitable

免疫原基因信息

human ... SPTLC1(10558)

免疫原蛋白记录号

O15269

外形

Unpurified

应用

Anti-SPTLC1 Antibody is an antibody against SPTLC1 for use in Western Blotting.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

其他说明

Concentration: Please refer to lot specific datasheet.

一般描述

Serine palmitoyltransferase 1 (EC 2.3.1.50; UniProt O15269; also known as LCB 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1) is encoded by the SPTLC1 (also known as HSAN1, HSAN1A, LCB1) gene (Gene ID 10558) in human. Serine palmitoyltransferase (SPT) catalyzes pyridoxal 5-phosphate-/PLP-dependent condensation of L-serine with palmitoyl-CoA to generate 3-ketodihydrosphingosine, the rate-limiting step for the de novo synthesis of sphingolipids. SPT belongs to the POAS (PLP-dependent α-oxoamine synthase) family of enzymes, members of this family also include 5-amino-levulinic acid synthase, KBL (2-amino-3 ketobutyrate ligase) and AONS (8-amino-7-oxononanoate synthase). Unlike the other known POAS family members that all exist as soluble homodimers, SPT is bound to the outer membrane of the endoplasmic reticulum (ER) as a multisubunit complex composed of three distinct subunits, SPTLC1, SPTLC2 and SPTLC3. SPTLC1 is a 473-amino acid sigle-transmembrane (a.a. 16-36) protein with a short N-terminal lumenal domain (a.a. 1-15) and a large C-terminal cytoplasmic domain (a.a. 37-473). Alternative splicing generates an isoform with a truncated cytoplasmic domain (D143 switched to E143, a.a. 144-473 truncated).

外形

Unpurified

免疫原

Two linear peptides corresponding to sequences from the N- and a C-terminal regions of human SPTLC1 cytoplasmic domain.

特异性

Expected to react with both spliced isoforms of human Serine palmitoyltransferase 1/SPTLC1 reported by UniProt (O15269).

储存及稳定性

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

质量

Evaluated by Western Blotting in HepG2 cell lysate.Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SPTLC1 in 10 µg of HepG2 cell lysate.

目标描述

~51 kDa observed. 52.74 kDa (isoform 1) and 16.07 kDa (isoform 2) calculated.