recombinant, expressed in HEK 293 cells
ZooMAb® learn more
primary antibodies
mouse, human
rabbit
3I12, recombinant monoclonal
IgG
expressed in HEK 293 cells
unconjugated
affinity binding assay: suitable;immunocytochemistry: suitable;immunohistochemistry: suitable;western blot: suitable
NP_001119580.1
human ... SLC12A3(6559)
recombinant expressionLearn more about Antibody Enhanced Validation
P55017
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Quality Control Testing Evaluated by Western Blotting in Mouse kidney tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SLC12A3 in Mouse kidney tissue lysate. Tested Applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected His-tagged Human SLC12A3 recombinant fragment. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC12A3 in HEK293 cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SLC12A3 in human kidney and mouse kidney tissue sections. Affinity Binding Assay: A representative lot of this antibody bound SLC12A3 peptide with a KD of 1.4 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.