| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 100.00 | 2.999 | 2.939 |
| 50.00 | 2.373 | 2.313 |
| 25.00 | 1.683 | 1.623 |
| 12.50 | 1.117 | 1.057 |
| 6.25 | 0.708 | 0.648 |
| 3.13 | 0.344 | 0.284 |
| 1.57 | 0.175 | 0.115 |
| 0.00 | 0.060 | 0.000 |
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-105% | 97% |
| EDTA plasma(n=5) | 95-110% | 100% |
| Heparin plasma(n=5) | 93-102% | 98% |
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-98% | 98-110% | 99-116% | 95-110% |
| EDTA plasma(n=5) | 87-102% | 85-95% | 85-98% | 82-95% |
| Heparin plasma(n=5) | 88-102% | 95-106% | 95-108% | 96-115% |
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