| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1600.00 | 0.211 | |
| 800.00 | 0.389 | |
| 400.00 | 0.655 | |
| 200.00 | 0.895 | |
| 100.00 | 1.119 | |
| 50.00 | 1.522 | |
| 25.00 | 1.762 | |
| 0.00 | 2.101 |
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-103% | 96% |
| EDTA plasma(n=5) | 82-95% | 88% |
| Heparin plasma(n=5) | 81-95% | 88% |
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-104% | 93-102% | 79-94% | 87-98% |
| EDTA plasma(n=5) | 87-98% | 92-105% | 95-107% | 82-96% |
| Heparin plasma(n=5) | 85-99% | 98-106% | 86-99% | 78-95% |
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